siRNA sequencing of virus-infected strawberry plant - SRA

SRX20250380: siRNA sequencing of virus-infected strawberry plant
1 ILLUMINA (Illumina NovaSeq 6000) run: 48.3M spots, 2.5G bases, 704.6Mb downloads

Design: Plant small RNA fraction was extracted with mirPremier microRNA Isolation Kit (SigmaAldrich, St. Louis, MI, USA) and processed with NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB, Ippswitch, MA, USA)

Submitted by: Biology Centre CAS

Study: Insight into host response, genomic interactions and insect vector-mediated transmission of viral lineages during co-infection of a single plant

PRJNA830324 • SRP371463 • All experiments • All runs

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Qualitative and quantitative viral population dynamics, host antiviral response, and host-to-host transmission of distinct genotypes using high throughput sequencing, aphids-mediated transmission assays. A single strawberry plant, hosting three different RNA viruses from Rhabdoviridae (strawberry crinkle virus, strawberry virus 1) and Secoviridae (strawberry mottle virus) families is used as a model.

Sample: plant infected with strawberry crinkle virus (Rhabdoviridae, 2 strains), strawberry mottle virus (Secoviridae, 3 RNA1 strains, 3 RNA2 strains), and strawberry virus 1 (Rhabdoviridae, 3 strains)

SAMN34995366 • SRS17583004 • All experiments • All runs

Organism: Fragaria x ananassa

Library:

Name: CRM-3K4

Instrument: Illumina NovaSeq 6000

Strategy: miRNA-Seq

Source: OTHER

Selection: size fractionation

Layout: SINGLE

Runs: 1 run, 48.3M spots, 2.5G bases, 704.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR24463537	48,319,892	2.5G	704.6Mb	2023-05-08

ID:: 27670826

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